Research Technologist 1

Northwestern University

Job Summary

Conducts tests and procedures; sets up and operates equipment; records observations and measurements and collects and compiles data in a research lab. Requires a practical understanding of a limited number of standard scientific techniques.

Key Responsibilities

• Follows pre-defined protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices.
• Conducts standard and repetitive experiments and completes basic trouble-shooting techniques.
• Sets-up, operates, and maintains standard lab equipment.
• Records test results and may maintain databases.
• Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
• Maintains inventory of reagents, mice, and lab supplies.
• Completes other duties as assigned.

Molecular Biology

• DNA, RNA, Protein isolation, purification and expression
• Southern, Northern, Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping, and sequencing of DNA
• Recombinant virus production and purification

Cellular Biology

• Cell culture, cell isolation
• Functional studies
• Transfection

Histology

• Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
• Immunohistochemistry
• Microscopy

Immunology

• Flow cytometry
• ELISA, Elispot assay
• Cytokine assay
• Antibody production and purification

Animal

• Husbandry
• Breeding and screening transgenic mice
• Dissections
• Blood collection
• Surgical procedures
• Database management of colony

Miscellaneous

• Perform other duties as assigned

Minimum Qualifications

• HS diploma or equivalent.
• Some basic science courses are required.