Associate Researcher I (Dr Dubois' Lab)

About the Role

The Dubois Laboratory at the Icahn School of Medicine at Mount Sinai investigates the fundamental mechanisms of cardiac development, with a focus on understanding how the heart is built at the cellular and molecular level. Research areas include cardiac progenitor specification, ventricular chamber morphogenesis, cardiomyocyte maturation, and congenital heart disease, utilizing mouse genetics, stem cell models, and genomic and imaging technologies.

Key Responsibilities

• Perform mammalian cell culture, including maintenance of pluripotent stem cell lines and differentiated cardiac cell types.
• Assist with mouse colony management, husbandry, and genotyping.
• Conduct standard molecular biology techniques including PCR, cloning, Western blotting, immunofluorescence, and qRT-PCR.
• Assist with tissue dissection, histology, and immunostaining on embryonic and adult cardiac tissue.
• Maintain detailed and organized laboratory records and experimental documentation.
• Contribute to the preparation of figures, protocols, and other materials supporting publications and grant applications.
• Participate actively in laboratory meetings and contribute to a collaborative team environment.
• Assist with general laboratory operations including ordering supplies and maintaining equipment.
• Performs other related duties.

Requirements & Tech Stack

• Mammalian cell culture (including pluripotent stem cell lines and differentiated cardiac cell types)
• Mouse colony management, husbandry, and genotyping
• PCR
• Cloning
• Western blotting
• Immunofluorescence
• qRT-PCR
• Tissue dissection
• Histology
• Immunostaining
• Confocal microscopy or other imaging modalities
• Flow cytometry/FACS
• Basic cell and molecular biology methods

Qualifications

• Bachelors or Masters degree in science or related field required.
• No experience required.
• One year of research experience preferred.

Highly Preferred Qualifications

• Hands-on experience with mammalian tissue culture techniques.
• Familiarity with basic cell and molecular biology methods.
• Experience with mouse husbandry, colony management, and genotyping.
• Prior exposure to developmental biology and/or cardiac biology.
• Experience with confocal microscopy or other imaging modalities.
• Familiarity with flow cytometry/FACS.
• Experience in a research laboratory setting beyond coursework.