Research Associate I, Merchant Lab

About the Role

The Merchant lab seeks a Research Associate with an interest in cancer biology and tumor immunology. Studies in the lab use mouse models to examine tumor immunology, with correlation in human patient samples. The studies are part of an NIH funded project aiming to increase our understanding of resistance of non-Hodgkin’s lymphoma (NHL) to chemotherapy and immunotherapy. The project will also examine the utility of CAR T-cells in the treatment of NHL. Results will be used to design clinical trials to improve outcomes for patients with NHL. Studies of the tumor microenvironment will be enabled using high dimensional flow cytometry, single cell RNA-sequencing, and imaging mass cytometry.

Key Responsibilities

• Breeding, genotyping, tumor injections, treatments, and tissue harvesting in mice.
• Apply standard molecular and cellular biology methods (RT-PCR, Western blotting, cell culture and transduction, expression profiling) to profile the tumor microenvironment of NHL.
• Uses imaging mass cytometry to study the tumor microenvironment and cell changes in animal models of NHL and in patient samples.
• Using flow cytometry to study the tumor microenvironment and cell changes in animal models of NHL and in patient samples.
• Maintains computer database with relevant information.
• Performs lab maintenance duties, including glassware cleaning and sterilization.
• Maintains stocks of general lab materials, and places orders for lab equipment and supplies.
• Assists new students and fellows at the laboratory.
• Assists in the operation of specialized equipment and machinery including mass cytometry system.
• Observes and complies with safety standards and procedures.
• Keeps accurate and detailed project records of experiments and results.

Opportunities

• Work with a diverse team, including other Research Associates, Medical Doctors, residents, graduate students, and post-doctoral students.
• Present data at the weekly Merchant Lab meeting.
• Help write papers.
• Attend national scientific meetings.
• Present data at national scientific meetings.

Requirements & Tech Stack

• Bachelor's Degree required.
• Interest in cancer biology and tumor immunology.
• Experience with mouse models.
• Experience with human patient samples.
• Experience with NIH funded projects.
• Experience with non-Hodgkin’s lymphoma (NHL).
• Experience with chemotherapy and immunotherapy.
• Experience with CAR T-cells.
• Experience with high dimensional flow cytometry.
• Experience with single cell RNA-sequencing.
• Experience with imaging mass cytometry.
• Experience with molecular and cellular biology methods:
• RT-PCR
• Western blotting
• Cell culture
• Transduction
• Expression profiling
• Experience with mass cytometry system.

This role is designed to be 100% onsite.