Associate Researcher I (Dr Dubois' Lab)

About the Role

The Dubois Laboratory at the Icahn School of Medicine at Mount Sinai investigates the fundamental mechanisms of cardiac development, focusing on cellular and molecular aspects of heart formation. Research areas include cardiac progenitor specification, ventricular chamber morphogenesis, cardiomyocyte maturation, and congenital heart disease, utilizing mouse genetics, stem cell models, and genomic and imaging technologies. The laboratory is described as a collaborative and collegial team.

Key Responsibilities

• Perform mammalian cell culture, including maintenance of pluripotent stem cell lines and differentiated cardiac cell types.
• Assist with mouse colony management, husbandry, and genotyping.
• Conduct standard molecular biology techniques including PCR, cloning, Western blotting, immunofluorescence, and qRT-PCR.
• Assist with tissue dissection, histology, and immunostaining on embryonic and adult cardiac tissue.
• Maintain detailed and organized laboratory records and experimental documentation.
• Contribute to the preparation of figures, protocols, and other materials supporting publications and grant applications.
• Participate actively in laboratory meetings and contribute to a collaborative team environment.
• Assist with general laboratory operations including ordering supplies and maintaining equipment.
• Perform other related duties.

Requirements & Tech Stack

• Mammalian cell culture techniques
• Mouse husbandry, colony management, and genotyping
• Basic cell and molecular biology methods (e.g., PCR, gel electrophoresis, Western blotting, immunostaining)
• Confocal microscopy or other imaging modalities
• Flow cytometry/FACS
• Developmental biology and/or cardiac biology
• Genomic and imaging technologies

Qualifications

• Bachelors or Masters degree in science or related field.
• No experience required.
• One year of research experience preferred.
• Experience in a research laboratory setting beyond coursework.